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Nucleic Acids Research, 1975, Vol. 2, No. 11 2007-2020
© 1975

Articles

Construction of a double-stranded deoxyribonucleotide sequence of 45 base pairs designed to code for S-peptide2-14 of bovine ribonuclease A

C.L. Harvey, K. Olson, A. de Czekala and A.L. Nussbaum

Chemical Research Department, Hoffmann-La Roche Inc. Nutley, NJ 07110, USA

Received July 30, 1975. An artificial DNA duplex, each strand consisting of 45 monomers, is constructed from chemically synthesized deoxyriboöligonucleotides. The resulting bihelical polymer may code for a modified S-peptide of Ribonuclease A. This is the first synthetic duplex designed to code for a eukaryotic message.


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