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Nucleic Acids Research, 1975, Vol. 2, No. 11 2069-2076
© 1975

Articles

Primary structure of tRNALys of E. coli B

Kalpana Chakraburtty, Assaf Steinschneider*, Richard V. Case+ and Alan H. Mehler

Department of Biochemistry, Medical College of Wisconsin Milwaukee, WI 53233, USA

Received August 27, 1975.

The primary structure of tRNALys of E. coli was determined by use of [32P]-tRNA. The sequence is pGGGUCGUUAGCUCAGDDGGDAGAGCAGUUGACUmam5-s2-UUU-t6AA{psi}CAAUUGm7GXCGCAGGT{psi}CGAAUCCUGCACGACCCACCA. No s4-U was detected in position 8. No other lysine tRNA was detected but the existence of another species has not been ruled out.

*Present address: Department of Biochemistry, University of Illinois Medical School, Chicago, Illinois

+Present address: P-L Biochemicals, Inc., Milwaukee, Wis.


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