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Nucleic Acids Research, 1975, Vol. 2, No. 12 2251-2256
© 1975

Articles

Isolation of mouse reticulocyte globin messenger ribonucleoprotein by affinity chromatography using oligo(T)-cellulose

Alan T.H. Burns and Robert Williamson

Institute of Animal Genetics, University of Edinburgh Edinburgh EH9 and Beatson Institute for Cancer Research Glasgow G3, UK

Received October 17, 1975. Mouse globin messenger ribonucleoprotein (mRNP) has been isolated from reticulocyte polysomes by affinity chromatography to oligo(T)-cellulose, using a procedure modified from that of Lindberg and Sundquist1. The messenger RNA and protein moieties of the mRNP are indistinguishable from those isolated by less rapid techniques, such as zonal ultracentrifugation.


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