Skip Navigation

This Article
Right arrow Print PDF (260K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Levy, A.
Right arrow Articles by Moav, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Levy, A.
Right arrow Articles by Moav, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1975, Vol. 2, No. 12 2299-2304
© 1975

Articles

Metrizamide density gradients of sea urchin chromatin: fractions rich and poor in nascent DNA

Abraham Levy, Karl M. Jakob and Boaz Moav*

Department of Plant Genetics, The Weizmann Institute of Science Rehovot *Department of Zoology, Tel Aviv University Ramat Aviv, Israel

Received November 3, 1975. A crude, lightly sheared chromatin preparation obtained from a mixture of[methyl-3H] thymidine pulse and [2-14C] thymidine long-labeled sea urchin embryos (swimming blastulae), was centrifuged in metrizamide to form an isopycnic gradient. The buoyant density of the 3H pulse labeled chromatin was slightly higher than that of the 14C labeled bulk chromatin. The 3H/14C ratios in the higher and lower density regions of the overlapping radioactivity peaks, indicated the presence of fractions rich and poor in nascent DNA in these two density regions. After 15 min chase, the difference disappeared, indicating that the chromatin fractions with nascent DNA have a half-life shorter than 15 min.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.