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Nucleic Acids Research, 1975, Vol. 2, No. 2 239-256
© 1975

Articles

In vivo and in vitro effects of rifampicin and streptolydigin on transcription of Kluyveromyces lactis in the presence of nystatin

P. Plevani, G. Badaracco{dagger}, N. Marmiroli* and G. Cassani+

Istituto di Genetica, Università di Pavia, and Laboratorio di Genetica Biochimica ed Evoluzionistica del CNR Pavia, Italy

Correspondence should be addressed to: Giovanni Cassani, Istituto di Genetica, Via S. Epifanio 14, 27100 Pavia, Italy

Received December 31, 1974.

Rifampicin and streptolydigin, if used in conjunction with nystatin, depress the growth of Kluyveromyces lactis. The incorporation of labeled leucine into protein is inhibited by nystatin whereas the incorporation of labeled uracil into RNA is inhibited by rifampicin in nystatin-treated cells. Fn order to study the mechanism of inhibition of RNA synthesis we purified by DEAE-Sephadex column chromatography four forms of RNA polymerase from K.lactis cells. The general properties of these enzymes are similar to those of Saccharomyces cerevisiae and of other eukaryotic RNA polymerases. In particular, enzymes IA, IB and III are more active with poly [d(A–T)] template and Mn2+ than with native or denatured calf thymus DNA. Enzyme II shows optimal activity with denatured calf thymus DNA and Mn2+. When challenged with native calf thymus DNA all enzymes prefer Mg2+ as a divalent cation whereas with denatured calf thymus DNA all enzymes are more active with Mn2+. Enzyme II is inhibited by {alpha}-amanitin but no enzyme is sensitive to rifampicin and streptolydigin. The inhibition of growth and uracil uptake observed when rifampicin is added to nystat in treated cells is probably not caused by a specific inhibition of transcription.

{dagger} Present address: Istituto di Fisiologia Generali, Università di Genova, Genova, Italy

* Present address: Istituto di Genetica, Università di Parma, Parma, Italy


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