Nucleic Acids Research, 1975, Vol. 2, No. 3 423-430
© 1975
Articles |
Preparation and separation of d(pT)10.n oligonucleotides
Department of Biochemistry, University of Bergen Bergen, Norway
Received February 13, 1975. A series of oligomers having the general formula d(pT)10.n varying from 2 to 20, has been prepared by enzymatic joining of d(pT)10, annealed on poly dA , employing TL polynucleotide ligase. The oligomers could be separated on 8 or 12% polyacrylamide gels. Sucv oligomers may prove useful as molecular weight markers and as initiators for various polymerases.
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