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Nucleic Acids Research, 1975, Vol. 2, No. 4 587-593
© 1975

Articles

Purification of SI nuclease from takadiastase by affinity chromatography on single-stranded DNA-acrylamide columns

Hanoch Slor

Department of Human Genetics, Sackler School of Medicine, Tel Aviv University Israel

Received March 4, 1975. When SI nuclease from Takadiastase was partially purified according to previously reported methods, it showed a 10 to 15 fold increase in specific-activity. Although such preparations were highly active on single-stranded DNA, they had traces of activity on native DNA and were contaminated by TI-RNase. The SI enzyme was further purified by a single step of affinity chromatography on single-stranded DNA-acrylamide column to a final purification of 275-fold. This preparation was free of Tl-RNase and had an absolute specificity for single-stranded DNA.


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