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Nucleic Acids Research, 1975, Vol. 2, No. 6 759-772
© 1975

Articles

Ionic strength effects on the stability and conformation of Penlctllium chrysogenum mycophage double stranded RNA

J. Paul Burnett, Bruce H. Frank and Richard J. Douthart*

The Lilly Research Laboratories Indianapolis, Indiana 46206, USA

Received February 25, 1975.

The effect of [NR+] on the stability and conformation of penicillium chrysogenum mycophage dsRNA (PCMdsRNA) was investigated using CD and UV optical techniques. Thermal melting profiles reveal prominent fine structure attributed to at least four regions of structural dissimilarity. A constant increased thermal stability of the dsRNA compared to DNA of the same base composition was observed over a oncentration range of 1.5 x 10–4 M to 4.5 x 10–2 M Na+. At low ionic strengths ([Na+]< 10–3 M) Tm becomes independent of further decreases in [Na+] unless the dsRNA is exposed to high concentrations of EDTA, suggesting the involvement of tightly bound divalent cation. At relatively high ionic strengths ([Na+] > 0.1 M) a postulated A – A' ... conformation change occurs.

*Author to whom correspondence should be addressed


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