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Nucleic Acids Research, 1975, Vol. 2, No. 6 831-838
© 1975

Articles

Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevistae

J.M. Schneller*, G. Faye**, C. Kujawa** and A.J.C. Stahl*

*Laboratoire de Biochimie, U.E.R.de Sciences pharmaceutiques, Université Louis Pasteur 3, rue de l'Argonne, 67083 Strasbourg **Centre de Génétique Moléculaire du C.N.R.S. 91190 Gif sur Yvette, France

Received March 24, 1975. Increasing amounts of mitochondrial [32P] tRNA (4S fraction) were hybridized with mitochondrial DNA of Saccharomyces cerevisiae. At saturation, the calculated number of genes for 4S mitochondrial RNA was 20. Mitochondrial [32P] tRNA eluted from the hydrids obtained either with an excess of tRNA or an excess of DNA showed, after alkaline hydrolysis and chromatography, a G + C content of 28 and 35 p. cent respectively. This last value is similar to that found with the total 4S fraction. The odd nucleotides T (about IT per sequence), U, hU are present in mitochondrial tRNA. Some sequences may begin with pG.


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