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Nucleic Acids Research, 1975, Vol. 2, No. 6 887-896
© 1975

Articles

The determination of secondary structure in the poly(C) tract of encephalomyocarditis virus RNA with sodium bisulphite

J. Goodchild, P. Fellner and A.G. Porter

Searle Research Laboratories Lane End Road, High Wycombe, Bucks., HP12 4HL, UK

Received April 14, 1975. The degree of secondary structure in the poly(C) tract of encephalomyocarditis virus (EMCV) RNA has been investigated using sodium bisulphite, which brings about the hydrolysis of non-base-paired cytidylic acid to uridylic acid in RNA. The percentage conversion of C to U in the poly(C) region of native EMCV RNA was similar to that found in a synthetic polynucleotide lacking secondary structure [poly(C)]. When poly(I) was annealed to either native or denatured EMCV RNA, it protected the poly(C) tract from the action of bisulphite. It is concluded that the poly(C) tract of EMCV RNA in solution is very largely singlestranded.


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