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Nucleic Acids Research, 1975, Vol. 2, No. 6 905-914
© 1975

Articles

Isolation of endonuclease from mammalian cells by DNA - cellulose chromatograpby

Antonio Caputo, Gabriella Zupi and Anna Clanciulli

Reglna Eiena Institute for Cancer Research Rome, Italy

Received April 21, 1975. By means of DNA-cellulose chromatography an enzyme with endonucleolytlc activity has been Isolated from nuclear acidic protein fraction of mammalian cells. The main active fraction, Bluted at 0.7 M NaCl, affects the velocity sedimentatian of UV-irradiated and alkylated DNA, resulting in a decrease of the molecular weight. The fraction is completely Inactive using native as well as heat-denatured DNA.


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