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Nucleic Acids Research, 1975, Vol. 2, No. 6 915-930
© 1975

Articles

Conversion of covalently mercurated nucleic acids to tritiated and halogenated derivatives

R.M.K. Dale, D.C. Ward, D.C. Livingston* and E. Martin**

Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine New Haven, Connecticut, 06510, U.S.A.

Received April 21, 1975.

Mercurated nucleic acids are converted to the corresponding tritiated, brominated, and iodinated derivatives by treatment with sodium borotritlide, N-bromosuccinimide, and elemental iodine, respectively. All three reactions occur under mild conditions in neutral aqueous solutions. Mercury-halogen conversions are essentially quantitative at both the monoand polynucleotide levels. Tritlation reactions also proceed efficiently with mononucleotides, although polymers undergo incomplete demercuration. In spite of the latter limitation, these reactions provide novel and efficient synthetic routes to radio labeled nucleic acid derivatives.

*D.C.L. was a recipient of an American Cancer Society-Eleanor Roosevelt Fellowship awarded by the International Union Against Cancer. Permanent address; Imperial Cancer Research Fund, Lincoln's Inn Fields, Londond, WC2, England.

**Present Address: Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio.


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