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Nucleic Acids Research, 1975, Vol. 2, No. 6 985-992
© 1975

Articles

Cyclization of uridine monophosphate by diethyl pyrocarbonate

F. Solymosy*, L. Ehrenberg** and I. Fedorcsak***

*Biological Research Center, Hungarian Academy of Sciences 6701 Szeged, P.O.Box 521, Hungary **Department of Radiobiology, University of Stockholm Wallenberg Laboratory, Lilla Frescati, 104 05 Stockholm, Eweden. ***Department of Genetics, Bötvös University Budapest, Hungary.

Received May 1, 1975. Reaction between diethyl pyrocarbonate and uridine 2'-phosphate or uridine 3'-phosphate leads to the formation in high yields of uridine 2' : 3'-cyclic phosphate. This reaction product was identified in experiments involving (a) ultraviolet spectrophotometry, (b) paper chromatography, (c) high voltage paper electrophoresis at both pH 3.5 and 7.4, (d) acid hydrolysis, and (e) digestion with pancreatic ribonuclease.


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