Nucleic Acids Research, 1975, Vol. 2, No. 7 1083-1092
© 1975
Articles |
Nucleotide composition analysis of tRNA from leukemia patient cell samples and human cell lines
Division of Biological Sciences, University of Missouri Columbia, Missouri 65201, USA
Received April 28, 1975. A technique developed for analysis of less than microgram quantities of tRNA has been applied to the study of human leukemia. Leucocytes from peripheal blood and bone marrow samples of six, untreated leukemia patients and cells of five different established human cell lines were maintained for 18 hours in media containing [32p]-phosphate. Incorporation of radioactive phosphate into the cells from the patient samples was slightly less than that of the cell lines. Likewise, incorporation of [32p]-phosphate into the tRNA of the patient samples (approximately 5 x 106 DPM/µg tRNA) was also less than that incorporated into the tRNA of the cell lines. The major and minor nucleotide compositions of the unfractionated tRNA preparations from each patient sample and each cell line were determined and compared. Similarities and differences in the major and minor nucleotide compositions of the tRNA preparations are discussed with reference to types of leukemia and the importance of patient sample analysis versus analysis of cultured human cells.