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Nucleic Acids Research, 1975, Vol. 2, No. 7 1153-1162
© 1975

Articles

Reversed phase chromatography of isoaccepting tRNA's from healthy and crown gall tissues from Nicotiana tabacum

P. Cornelis, E. Claessen and J. Claessen

Laboratoire de Cytogénétique, Institut Carnoy 4, place de la Croix du Sud, 1348 Louvain-la Neuve, Belgium

Received May 20, 1975. RPC 5 (Reversed Phase Chromatography)1 of aminoacyl-tRNA's from healthy and crown gall (induced by Agrobacterium tumefaciens strain B6) tobacco tissues were compared for eleven amino acids.

For ten amino acids: alanine,arginine,glutamic acid,glycine, isoleucine,leucine,lysine.methionine.tyrosine,and valine, no qualitative or quantitative differences could be detected between aminoacyl-tRNA's from both sources.

Phenylalanyl-tRNA's from crown gall tissues gave two peaks on RPC 5;the minor early eluting species (peak 1) was always absent in elution profiles of phenylalanyl-tRNA's from healthy tissues or from tobacco leaves.

After the "Y" base was removed by pH 2.9 treatment2 ,peak 2 of phenylalanine tRNA was shifted to the position of peak 1.


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