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Nucleic Acids Research, 1975, Vol. 2, No. 7 1163-1176
© 1975

Articles

Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters

Ronald W. Pero, Tomas Bryngelsson, Carl Bryngelsson, Adam Deutsch, Åke Nordén2 and Allan Norgren3

Department of Biochemistry, University of Lund Box 740, 220 07 Lund, Sweden 2Haematology Laboratory, University Hospital Lund, Sweden 3Department of Radiation Therapy, University Hospital Lund, Sweden

Received May 20, 1975. The level of deoxyadenylate (dA) regions in human DNA was estimated from formation of poly(U)-poly(dA) triplexes on nitrocellulose filters that were RNAase resistant. The (dA) rich sequences were determined following mild ribonuclease treatment of the poly(U)-DNA hybrids (5 µg/ml at 25°C for 30 min), where as exhaustive ribonuclease treatment (5 µg/ml at 25°C for 6 hr) estimated the more (dA) pure sequences. The level of (dA) rich regions was 0.39% of the DNA and for the more (dA) pure regions it was 0.07%.

The (dA) regions were widely distributed throughout human DNA regardless of base composition or sequence repetition. However, a concentration of (dA) regions into main band CsCl gradient fractions of DNA and into repeated DNA was observed.


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