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Nucleic Acids Research, 1975, Vol. 2, No. 8 1237-1260
© 1975

Articles

A chemical approach to studies of the three-dimensional structure of tRNA - alkylation with a reagent covalently bound to a ‘peculiar site’

M.A. Grachev and M.I. Rivkin

Institute of Organic Chemistry Novosibirsk 90, USSR

Received April 17, 1975. Yeast valine tRNA1 was chemically modified with chlorambucil N-hydroxysuccinimide ester. The reagent was attached covalently to the valine residue of valyl-tRNA1Val under the conditions which prevented tRNA from alkylation. Chlorambucilyl-valyl-tRITA1Val thus obtained was separated from excess reagent and incubated in an aqueous solution at neutral pH in the presence of Mg++ ions. Highly efficient intramolecular Val self-alkylation of chlorambucilyl-valyl-tRNA1Val took place. The chlorambucil residue bound covalently to the amino group of the valine residue of tRNA1Valalkylates the 5'-terminal phosphate group of the molecule, and its 3'-terminal sequence -A-C-C-A.


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