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Nucleic Acids Research, 1975, Vol. 2, No. 8 1291-1304
© 1975

Articles

Drosophila chromatin: an immunological study

David B. Roberts and Peter W. Andrews*

Genetics Laboratory, Biochemistry Department South Parks Road, Oxford, UK

*Laboratoire de Genetique Cellulaire, Institut Pasteur et College de France 25 rue du Dr. Roux, 75015 Paris, France

Received May 23, 1975. Antibodies were prepared against chromatin, various chromosomal protein preparations and against cytoplasm from Drosophila larvae. These antibodies were used to study the distribution of antigens in chromatin and chromosomal protein preparations on double diffusion plates. Antisera from all of the mammals tested precipitated both chromatin and DNA on double diffusion plates run in water. This non-specific precipitation was removed by washing in 0.06M NaCl.


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