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Nucleic Acids Research, 1975, Vol. 2, No. 8 1381-1390
© 1975

Articles

DNA replication in Physarum polycephalum: characterization of replication products in vivo

Steinar Funderud and Finn Haugli

Institute of Medical Biology, University of Troms{phi} Troms{phi}, Norway

Received June 30, 1975. Synchronous plasmodia of Physarum polycephalum in DNA synthesis were pulse-labelled with [3H] - thymidine for timeperiods of 15 seconds up to 9 minutes, or given a 30 seconds pulse followed by chase periods of 9 minutes up to 6 hours. Sedimentation analysis in alkaline sucrose gradients revealedat least five species of single stranded DNA hinolecules in the pulse experiments. Co-sedimentation of [14C]-labelled phage-DNA gave relative S-values of 5–7, 13–15, 23–25, 30 and 33–35 for these DNA molecules, all of which can be chased into DNA of higher molecular weight.


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