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Nucleic Acids Research, 1975, Vol. 2, No. 8 1421-1431
© 1975

Articles

tRNA tertiary structure in solution as probed by the photochemically induced 8-13 cross-link

A. Favre+, R. Buckingham* and G. Thomas+

+Institut de Biologie Moleculaire du CNRS et de l'Université Paris VII, 75005 Paris, France *Institut de Biologie Physico-Chimique 13 rue Pierre et Marie Curie, 75005 Paris, France

Received July 4, 1975. The conformation of ten purified tRNAs from Escherichia Coli has been investigated by means of the photo-induced cross-linking of 4Srd8 and Cydl3, which is sensitive to the juxtaposition of the two bases. Three tRNIs photoreact abnormally slowly, tRNAPhe, tRNAmMet and tRNA2Val ; a comparison with normally reacting species suggests that base 47 (Urd or modified Urd) is involved in a tertiary interaction in Class I tRNAs with the triplet 8, 14, 28.

The UGA suppressor tRNATrp photoreacts significantly slower than the wild type. Thus the single base change Gua 24 to Ade induces a conformational change that alters the rate constant for the cross-linking reaction.


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