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Nucleic Acids Research, 1975, Vol. 2, No. 9 1513-1524
© 1975

Articles

Misincorporation of GTP during transcription of poly dAT-dAT and poly dABU-dABU

Michael D. Bick

Roche Institute of Molecular Biology Nutley, NJ 07110, USA

Received June 6, 1975. The synthetic DNA alternating copolymers poly dAT-dAT and dABU-dABU have been transcribed with E. coli RNA polymerase to measure the level of BrdUinduced misincorporation of guanine during transcription. GTP is found to be misincorporated into both copolymers at a frequency of 1 per 1000-2000 nucleotides polymerized. Using {alpha}–32p-GTP, the nearest neighbors to GMP are found to be UMP (~63%), GMP (~25%) and AMP (~17%), with no apparent difference between the two templates. These results suggest that BrdU-substitution in DNA does not necessarily increase the potential for base mispairing during transcription, and hence, promote the production of faulty RNA molecules.


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