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Nucleic Acids Research, 1975, Vol. 2, No. 9 1525-1538
© 1975

Articles

Heterogeneity of chromatin fragments produced by micrococcal nuclease action

R.L. Rill*, D.K. Oosterhof, J.C. Hozier and D.A. Nelson

Department of Chemistry and Institute of Molecular Biophysics, The Florida State University Tallahassee, FL 32306, USA

*Author to whom reprint requests should be sent.

Received June 6, 1975. Digestion of calf thymus chromatin with micrococcal nuclease produces a mixture of apparently well defined nucleoprotein fragments which have been partially resolved by sedimentation on linear (5-20%) sucrose gradients. Sedimentation patterns reveal a predominent peak at the 11s position; three slower components, which have not previously been reported, at the 3.4S, 5.3S and 8.6S positions, and three faster components at the 17S, 22s and 26S positions. DNA isolated from the 3S to 12S region of gradients has been resolved on polyacrylamide gels into nine to ten discrete components ranging from 47 to 156 base pairs in length. A nearly identical pattern of small DNA products was obtained from chromatin digested in intact nuclei. These data suggest that chromatin contains either several types of subunits or predominently a single type of subunit which can be asymmetrically cleaved at any one of four or more sites.


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C. Hanson, C. Shen, and J. Hearst
Cross-linking of DNA in situ as a probe for chromatin structure
Science, July 2, 1976; 193(4247): 62 - 64.
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