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Nucleic Acids Research, 1975, Vol. 2, No. 9 1609-1620
© 1975

Articles

RNA nicking activity associated with DNA ligase of T4 infected E. coli: properties and influence on in vitro reactions of ligase.

Hiroshi Sano and Günter Feix

Institut für Biologie III (Genetik, Molekularbiologie und Biophysik), Universitat Freiburg 78 Freiburg i.Br., West-Germany

Received July 22, 1975. Highly purified DNA ligase from T4 infected E.coli displays an RNA nickingactivity which cleaves endonucleolytically the RNA of ribo-desoxy-and riboribo type doublestranded structures to oligonucleotides with 5'phosphoryland 3'hydroxy termini. In the presence of ATP the generated nicks are repaired by the ligase except at the ends of the double stranded regions where some short oligonucleotides are released before ligation can occur. As judged from its behaviour during the various purification steps and from some of its properties, the nicking activity seems to be different from known nicking enzymes.


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