Skip Navigation

This Article
Right arrow Print PDF (3192K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (75)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Murchie, A. I.H.
Right arrow Articles by Lilley, D. M.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murchie, A. I.H.
Right arrow Articles by Lilley, D. M.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 1 49-53
© 1992

MOLECULAR BIOLOGY

Retinoblastoma susceptibility genes contain 5' sequences with a high propensity to form guanine-tetrad structures

Alastair I.H. Murchie and David M.J. Lilley*

Department of Biochemistry, The University Dundee DD1 4HN, UK

*To whom correspondence should be addressed

Received October 28, 1991. Revised December 3, 1991. Accepted December 3, 1991.

Retinoblastoma susceptibility genes contain significant runs of oligoguanine at their 5' ends. Oligonucleotides having these sequences underwent complex formation in the presence of sodium ions, in which there was association of four strands. Formation of this structure was completely prevented of guanine was replaced by 7-deazaguanine, indicating the importance of guanine N7 in the formation of the complex. Complex formation lead to protection of guanine N7 against methylation by dimethyl sulphate, but thymine bases located between oligoguanine blocks were reactive to osmium tetroxide. There was also some sensitivity to S1 nuclease to the 5' side of the oligoguanine block. The results show that the G-rich regions of the mouse and human retinoblastoma susceptibility genes have a propensity to undergo tetraplex formation of the kind demonstrated in the immunoglobulin switch region.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Paramasivam, S. Cogoi, V. V. Filichev, N. Bomholt, E. B. Pedersen, and L. E. Xodo
Purine twisted-intercalating nucleic acids: a new class of anti-gene molecules resistant to potassium-induced aggregation
Nucleic Acids Res., June 1, 2008; 36(10): 3494 - 3507.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. K. Yadav, J. K. Abraham, P. Mani, R. Kulshrestha, and S. Chowdhury
QuadBase: genome-wide database of G4 DNA occurrence and conservation in human, chimpanzee, mouse and rat promoters and 146 microbes
Nucleic Acids Res., January 11, 2008; 36(suppl_1): D381 - D385.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. A. Rachwal, I. S. Findlow, J. M. Werner, T. Brown, and K. R. Fox
Intramolecular DNA quadruplexes with different arrangements of short and long loops
Nucleic Acids Res., June 18, 2007; (2007) gkm316v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Xu and H. Sugiyama
Formation of the G-quadruplex and i-motif structures in retinoblastoma susceptibility genes (Rb)
Nucleic Acids Res., February 7, 2006; 34(3): 949 - 954.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
E. E. Merkina and K. R. Fox
Kinetic Stability of Intermolecular DNA Quadruplexes
Biophys. J., July 1, 2005; 89(1): 365 - 373.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
W. Duan, A. Rangan, H. Vankayalapati, M.-Y. Kim, Q. Zeng, D. Sun, H. Han, O. Yu. Fedoroff, D. Nishioka, S. Y. Rha, et al.
Design and Synthesis of Fluoroquinophenoxazines That Interact with Human Telomeric G-Quadruplexes and Their Biological Effects
Mol. Cancer Ther., December 1, 2001; 1(2): 103 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Lew, W. J. Rutter, and G. C. Kennedy
Unusual DNA structure of the diabetes susceptibility locus IDDM2 and its effect on transcription by the insulin promoter factor Pur-1/MAZ
PNAS, November 7, 2000; 97(23): 12508 - 12512.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. J. Bates, J. B. Kahlon, S. D. Thomas, J. O. Trent, and D. M. Miller
Antiproliferative Activity of G-rich Oligonucleotides Correlates with Protein Binding
J. Biol. Chem., September 10, 1999; 274(37): 26369 - 26377.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Simonsson and R. Sjoback
DNA Tetraplex Formation Studied with Fluorescence Resonance Energy Transfer
J. Biol. Chem., June 11, 1999; 274(24): 17379 - 17383.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Tzfati, H. Abeliovich, D. Avrahami, and J. Shlomai
Universal Minicircle Sequence Binding Protein, a CCHC-type Zinc Finger Protein That Binds the Universal Minicircle Sequence of Trypanosomatids
J. Biol. Chem., September 8, 1995; 270(36): 21339 - 21345.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.