Skip Navigation

This Article
Right arrow Print PDF (2171K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Schmidt, S.
Right arrow Articles by Cech, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmidt, S.
Right arrow Articles by Cech, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 10 2421-2426
© 1992

CHEMISTRY

Chemical synthesis of 2'-deoxyoligonucleotides containing 5-fluoro-2'-deoxycytidine

Sabine Schmidt, Claus-Dietmar Pein+, Hans-Joachim Fritz1,* and Dieter Cech

Institut für Bioorganische Chemie, Humboldt-Universität zu Berlin Invalidenstraße 42, O-1040 Berlin 1Institut für Molekulare Genetik, Georg-August-Universität Göttingen Grisebachstraße 8, W-3400 Göttingen, Germany

* To whom correspondence should be addressed

Received April 8, 1992. Accepted April 22, 1992.

2'-Deoxyoligonucleotides with 5-fluorocytosine residues incorporated at specific positions of the nucleotide sequence are tools of great potential in the study of the catalytic mechanism by which DNA cytosine methyltransferases methylate the 5-position of DNA cytosine residues in specific sequence contexts. Chemical synthesis of such oligonucleotides is described. Two alternative approaches have been developed, one of which proceeds via a fully protected phosphoramidite of 5-fluoro-4-methylmercapto-2'-deoxyuridine 2, the other via a fully protected phosphoramidite of 5-fluoro-2'-deoxycytidine 3. Either building block can be used in automated oligonucleotide synthesis applying standard elongation cycles and deprotection procedures exclusively. The methylmercapto function of 2 is replaced by an amino group in the final ammonia treatment used for cleavage from support and base deprotection.

+ Present address: Pharmacia Biosystems GmbH, Munzinger Straße 9, W-7800 Freiburg, Germany


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
C. Schmidt, R. Welz, and S. Muller
RNA double cleavage by a hairpin-derived twin ribozyme
Nucleic Acids Res., February 15, 2000; 28(4): 886 - 894.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.