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Nucleic Acids Research, 1992, Vol. 20, No. 10 2435-2439
© 1992

CHEMISTRY

Solid-phase synthesis of an RNA nucleopeptide fragment from the nucleoprotein of poliovirus

C.M. Dreef-Tromp, H. van den Elst, J.E. van den Boogaart, G.A. van der Marel and J.H. van Boom*

Gorlaeus Laboratory PO Box 9502, 2300 RA Leiden, The Netherlands

* To whom correspondence should be addressed

Received March 18, 1992. Revised April 21, 1992. Accepted April 21, 1992.

The naturally occurring RNA-nucleopeptide H-Ala-Tyr[5'-pUUAAAAC-3']-NH2 is prepared via a solid-phase phosphite triester approach using N-SiOMB/O-TBDMS-protected nucleosides. Preliminary 1H-NMR studies show that the peptidyl unit has a remarkable effect on the conformational behaviour of the RNA moiety in the nucleopeptide.


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