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Nucleic Acids Research, 1992, Vol. 20, No. 10 2447-2452
© 1992

MOLECULAR BIOLOGY

Field inversion gel electrophoresis in denaturing polyacrylamide gels

Christoph Heller* and Stephan Beck

Imperial Cancer Research Fund 44 Lincoln's Inn Fields, London WC2A 3PX, UK

* To whom correspondence should be addressed at Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris, Laboratoire de Physico-Chimie Théorique, 10 rue Vauquelin, 75231 Paris cedex 05, France

Received March 5, 1992. Revised April 16, 1992. Accepted April 16, 1992.

The velocities of single stranded DNA molecules in denaturing polyacrylamide gels during symmetric and asymmetric field inversion were measured at different pulse times and gel concentrations. Under the conditions chosen in our study, pulse times as short as a few milliseconds lead to a retardation of DNA molecules larger than 400 bases. We found that a field inversion with an electric field in the forward direction of about double the strength of that applied in the backward direction is a good compromise between the degree of retardation, the temperature control requirements and the run time of the gel.


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