Nucleic Acids Research, 1992, Vol. 20, No. 10 2485-2492
© 1992
MOLECULAR BIOLOGY |
Mapping functional regions of the segment-specific transcription factor Krox-20
Laboratoire de Génétique Moléculaire CNRS D 1302, Ecole Normale Supérieure, 46 rue d'Ulm, F-75230 Paris Cedex 05, France
* To whom correspondence should be addressed
Received February 7, 1992. Revised April 10, 1992. Accepted April 10, 1992.
Krox-20, a zinc finger transcription factor with similarity to Sp1, is likely to play an important role in the development of the vertebrate central nervous system. A knowledge of its molecular properties will help to understand its physiological functions. We have therefore performed a structure-function analysis of the protein to identify the regions involved in DNA-binding and transcriptional activation. Our data suggest that only the zinc fingers are required for high affinity, specific DNA-binding. Transcriptional activation was not affected by deletion of the C-terminal tail of the protein. In contrast, deletion of the N-terminal half, upstream of the zinc fingers, completely abolished transactivation without affecting DNA-binding or nuclear localization. Two transcriptional activation domains were identified in this region. They cooperate to establish full activity. They are rich in negatively-charged amino acids and are therefore may constitute acidic activation domains. Comparative analysis of the amino acid sequences of several zinc finger proteins belonging to the Krox-20 subfamily indicates that they contain acidic regions at similar locations within their N-terminal region, suggesting that the functional organization of these proteins has been conserved during evolution.
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