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Nucleic Acids Research, 1992, Vol. 20, No. 10 2591-2596
© 1992

MOLECULAR BIOLOGY

Transcriptional activity of the human immunodeficiency virus-1 LTR promoter in fission yeast Schizosaccharomyces pombe

Reiko Toyama, Steven M. Bende+ and Ravi Dhar*

Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA

* To whom correspondence should be addressed

Received September 6, 1991. Revised April 6, 1992. Accepted April 6, 1992.

We have analyzed the transcriptional activity of the human immunodeficiency virus type I (HIV-1) LTR promoter in the fission yeast Schizosaccharomyces pombe (S.pombe). The ability of a series of 5'-deleted forms of the HIV-1 LTR promoter to direct transcription of the chloramphenicol acetyltransferase reporter gene was studied. We found that the HIV-1 promoter is functional in S.pombe and that deletion of sequences upstream of the NF-kB binding site previously identified to contain the negative regulatory element (NRE) in mammalian cells, resulted in an about thirty-fold increase in transcriptional activity. Sequences in the HIV-1 promoter that bind NF-kB were found to be essential for transcriptional activation in S.pombe. In mammalian cells, transactivation of the HIV-1 LTR requires TAR sequences and the viral Tat protein. In fission yeast, Tat failed to transactivate the HIV-1 LTR, suggesting that S.pombe may lack a cellular factor(s) required for the Tat transactivation process.

+ Present address: Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA


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[Abstract] [Full Text] [PDF]


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