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Nucleic Acids Research, 1992, Vol. 20, No. 11 2667-2671
© 1992

GENOME STRUCTURE AND MAPPING

An African swine fever virus gene with homology to DNA ligases

Jef M. Hammond, Shona M. Kerr1,+, Geoffrey L. Smith1 and Linda K. Dixon*

AFRC Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey GU24 0NF 1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK

*To whom correspondence should be addressed

Received April 8, 1992. Revised April 30, 1992. Accepted April 30, 1992.

Sequence analysis of the Sa/I g region of the genome of a virulent isolate of ASFV (Malawi LII 20/1) has revealed an open reading frame with the potential to encode a 48 kllodalton (kD) polypeptide which has significant homology with eukaryotic and prokaryotic DNA ligases. This ASFV encoded gene also contains the putative active site region of DNA ligases including the lysine residue which is necessary for enzyme adenylate adduct formation, but lacks the C-terminal basic region conserved in other eukaryotic DNA ligases. A novel [32P]-labelled potential DNA ligase adenylate adduct of Mr 45 kD was observed upon incubation of ASFV infected cell cytoplasmic extracts with {alpha}[32]- and subsequent analysis of products by SDS/PAGE. These data together suggest that ASFV encodes its own DNA ligase.

+Present address: Structural Studies Section, MRC Human Genetics Unit. Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK


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