Skip Navigation

This Article
Right arrow Print PDF (526K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (50)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pisani, F. M.
Right arrow Articles by Rossi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pisani, F. M.
Right arrow Articles by Rossi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 11 2711-2716
© 1992

MOLECULAR BIOLOGY

A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases

Francesca M. Pisani, Ciro De Martino and Mosè Rossi*

Istituto di Biochimica delle Proteine ed Enzimologia, Consiglio Nazionale Ricerche, Via Marconi 10, 80125, Naples, Italy

*To whom correspondence should be addressed

Received March 18, 1992. Revised May 7, 1992. Accepted May 7, 1992.

The gene encoding the thermostable DNA polymerase from the archaeon Sulfolobus solfataricus (strain MT 4) was isolated by means of two degenerate oligonucleotide probes. They were designed on the basis of partial enzyme amino acid sequences. The gene was found to encode a 882 residues polypeptide chain with a deduced molecular mass of about 100 kDa. By comparison with other archaeal genes, putative regulatory sites were identified in the gene-flanking regions. By computer-assisted homology search, several sequence similarities among S.solfataricus and family B DNA polymerases were found. In addition, conserved sequence motifs,implicated in the 3'–5' exonuclease activity of E.coli DNA polymerase I and shared by various family A and B DNA polymerases, were also identified. This result suggests that the proofreading domains of all these enzymes are evolutionarily related.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. Lou, Z. Duan, X. Huo, and L. Huang
Modulation of Hyperthermophilic DNA Polymerase Activity by Archaeal Chromatin Proteins
J. Biol. Chem., January 2, 2004; 279(1): 127 - 132.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Gruz, F. M. Pisani, M. Shimizu, M. Yamada, I. Hayashi, K. Morikawa, and T. Nohmi
Synthetic Activity of Sso DNA Polymerase Y1, an Archaeal DinB-like DNA Polymerase, Is Stimulated by Processivity Factors Proliferating Cell Nuclear Antigen and Replication Factor C
J. Biol. Chem., December 7, 2001; 276(50): 47394 - 47401.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. P. Villarreal and V. R. DeFilippis
A Hypothesis for DNA Viruses as the Origin of Eukaryotic Replication Proteins
J. Virol., August 1, 2000; 74(15): 7079 - 7084.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
M. Kähler and G. Antranikian
Cloning and Characterization of a Family B DNA Polymerase from the Hyperthermophilic Crenarchaeon Pyrobaculum islandicum
J. Bacteriol., February 1, 2000; 182(3): 655 - 663.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
I. K. O. Cann, S. Ishino, N. Nomura, Y. Sako, and Y. Ishino
Two Family B DNA Polymerases from Aeropyrum pernix, an Aerobic Hyperthermophilic Crenarchaeote
J. Bacteriol., October 1, 1999; 181(19): 5984 - 5992.
[Abstract] [Full Text]

Home page
 GeneticsHome page
I. K. O. Cann and Y. Ishino
Archaeal DNA Replication: Identifying the Pieces to Solve a Puzzle
Genetics, August 1, 1999; 152(4): 1249 - 1267.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
I. K. O. Cann, K. Komori, H. Toh, S. Kanai, and Y. Ishino
A heterodimeric DNA polymerase: Evidence that members of Euryarchaeota possess a distinct DNA polymerase
PNAS, November 24, 1998; 95(24): 14250 - 14255.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.