Nucleic Acids Research, 1992, Vol. 20, No. 11 2813-2817
© 1992
MOLECULAR BIOLOGY |
Promoter specificity of the two transcriptional activation functions of the human oestrogen receptor in yeast
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Genétique de I'INSERM, Institut de Chimie Biologique, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cédex, France
*To whom correspondence should be addressed
Received February 7, 1992. Revised April 30, 1992. Accepted April 30, 1992.
We have previously demonstrated that the human oestrogen receptor (hER) contains two transcriptional activation functions located in the N-terminal region (TAF-1) and in the hormone binding domain (TAF-2), which can act both independently and synergistically in a promoter-and cell-specific manner in animal cells. We have also demonstrated that hER can activate transcription from chimaeric oestrogen-responsive GAL1 promoters in yeast, and shown that transcriptional activation was due to TAF-1, whereas TAF-2 showed little, if any, transcriptional activity on these promoters. By using a more complex promoter derived from the URA3 gene, we now show that TAF-2 is also active in yeast, and that the activities of TAF-1 and TAF-2 are promoter-context-specific in yeast. We also confirm that the agonistic activity of 4-hydroxytamoxifen (OHT) can be ascribed to the activity of TAF-1.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. S Thomas, N. Sarwar, F. Phoenix, R C. Coombes, and S. Ali Phosphorylation at serines 104 and 106 by Erk1/2 MAPK is important for estrogen receptor-{alpha} activity J. Mol. Endocrinol., April 1, 2008; 40(4): 173 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Martin, M. B. Taylor, G. Krikun, C. Lockwood, G. E. Akbas, and H. S. Taylor Differential Cell-Specific Modulation of HOXA10 by Estrogen and Specificity Protein 1 Response Elements J. Clin. Endocrinol. Metab., May 1, 2007; 92(5): 1920 - 1926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Germain, B. Staels, C. Dacquet, M. Spedding, and V. Laudet Overview of Nomenclature of Nuclear Receptors Pharmacol. Rev., December 1, 2006; 58(4): 685 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. K. Keeton and M. Brown Cell Cycle Progression Stimulated by Tamoxifen-Bound Estrogen Receptor-{alpha} and Promoter-Specific Effects in Breast Cancer Cells Deficient in N-CoR and SMRT Mol. Endocrinol., June 1, 2005; 19(6): 1543 - 1554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Oltra, I. Pfeifer, and R. Werner Ini, a Small Nuclear Protein that Enhances the Response of the Connexin43 Gene to Estrogen Endocrinology, July 1, 2003; 144(7): 3148 - 3158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. H. Coulthard, S. Matsuda, and D. M. Heery An Extended LXXLL Motif Sequence Determines the Nuclear Receptor Binding Specificity of TRAP220 J. Biol. Chem., March 21, 2003; 278(13): 10942 - 10951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shang and M. Brown Molecular Determinants for the Tissue Specificity of SERMs Science, March 29, 2002; 295(5564): 2465 - 2468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Metivier, G. Penot, G. Flouriot, and F. Pakdel Synergism Between ER{alpha} Transactivation Function 1 (AF-1) and AF-2 Mediated by Steroid Receptor Coactivator Protein-1: Requirement for the AF-1 {alpha}-Helical Core and for a Direct Interaction Between the N- and C-Terminal Domains Mol. Endocrinol., November 1, 2001; 15(11): 1953 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Métivier, F. G. Petit, Y. Valotaire, and F. Pakdel Function of N-Terminal Transactivation Domain of the Estrogen Receptor Requires a Potential {alpha}-Helical Structure and Is Negatively Regulated by the A Domain Mol. Endocrinol., November 1, 2000; 14(11): 1849 - 1871. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. G. Petit, Y. Valotaire, and F. Pakdel The analysis of chimeric human/rainbow trout estrogen receptors reveals amino acid residues outside of P- and D-boxes important for the transactivation function Nucleic Acids Res., July 15, 2000; 28(14): 2634 - 2642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ha, K. Hellauer, and B. Turcotte Fusions with histone H3 result in highly specific alteration of gene expression Nucleic Acids Res., February 15, 2000; 28(4): 1026 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Pakdel, R. Metivier, G. Flouriot, and Y. Valotaire Two Estrogen Receptor (ER) Isoforms with Different Estrogen Dependencies Are Generated from the Trout ER Gene Endocrinology, February 1, 2000; 141(2): 571 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Bevan, S. Hoare, F. Claessens, D. M. Heery, and M. G. Parker The AF1 and AF2 Domains of the Androgen Receptor Interact with Distinct Regions of SRC1 Mol. Cell. Biol., December 1, 1999; 19(12): 8383 - 8392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang and J. D. Fondell Identification of Mouse TRAP100: a Transcriptional Coregulatory Factor for Thyroid Hormone and Vitamin D Receptors Mol. Endocrinol., July 1, 1999; 13(7): 1130 - 1140. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. S. Hunter, L. C. Hodges, P. M. Vonier, R. Fuchs-Young, M. M. Gottardis, and C. L. Walker Estrogen Receptor Activation via Activation Function 2 Predicts Agonism of Xenoestrogens in Normal and Neoplastic Cells of the Uterine Myometrium Cancer Res., July 1, 1999; 59(13): 3090 - 3099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hecht, C. M. Litterst, O. Huber, and R. Kemler Functional Characterization of Multiple Transactivating Elements in beta -Catenin, Some of Which Interact with the TATA-binding Protein in Vitro J. Biol. Chem., June 18, 1999; 274(25): 18017 - 18025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Knoblauch and M. J. Garabedian Role for Hsp90-Associated Cochaperone p23 in Estrogen Receptor Signal Transduction Mol. Cell. Biol., May 1, 1999; 19(5): 3748 - 3759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Milocco, J. A. Haslam, J. Rosen, and H. M. Seidel Design of Conditionally Active STATs: Insights into STAT Activation and Gene Regulatory Function Mol. Cell. Biol., April 1, 1999; 19(4): 2913 - 2920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Stafford and R. H. Morse Mutations in the AF-2/Hormone-binding Domain of the Chimeric Activator GAL4·Estrogen Receptor·VP16 Inhibit Hormone-dependent Transcriptional Activation and Chromatin Remodeling in Yeast J. Biol. Chem., December 18, 1998; 273(51): 34240 - 34246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fraser, D. J. Heard, S. Adam, A. C. Lavigne, B. Le Douarin, L. Tora, R. Losson, C. Rochette-Egly, and P. Chambon The Putative Cofactor TIF1alpha Is a Protein Kinase That Is Hyperphosphorylated upon Interaction with Liganded Nuclear Receptors J. Biol. Chem., June 26, 1998; 273(26): 16199 - 16204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. vom Baur, M. Harbers, S.-J. Um, A. Benecke, P. Chambon, and R. Losson The yeast Ada complex mediates the ligand-dependent activation function AF-2 of retinoid X and estrogen receptors Genes & Dev., May 1, 1998; 12(9): 1278 - 1289. [Abstract] [Full Text]
|
|
|
|
|
|
W. Porter, B. Saville, D. Hoivik, and S. Safe Functional Synergy between the Transcription Factor Sp1 and the Estrogen Receptor Mol. Endocrinol., October 1, 1997; 11(11): 1569 - 1580. [Abstract] [Full Text]
|
|
|
|
|
|
J. R. Wilkinson and H. C. Towle Identification and Characterization of the AF-1 Transactivation Domain of Thyroid Hormone Receptor beta 1 J. Biol. Chem., September 19, 1997; 272(38): 23824 - 23832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nichols, J. M. J. Rientjes, C. Logie, and A. F. Stewart FLP Recombinase/Estrogen Receptor Fusion Proteins Require the Receptor D Domain for Responsiveness to Antagonists, but not Agonists Mol. Endocrinol., June 1, 1997; 11(7): 950 - 961. [Abstract] [Full Text]
|
|
|
|
|
|
A. Joyeux, V. Cavaillès, P. Balaguer, and J. C. Nicolas RIP 140 Enhances Nuclear Receptor-Dependent Transcription in Vivo in Yeast Mol. Endocrinol., February 1, 1997; 11(2): 193 - 202. [Abstract] [Full Text]
|
|
|
|
|
|
E. M. McInerney and B. S. Katzenellenbogen Different Regions in Activation Function-1 of the Human Estrogen Receptor Required for Antiestrogen- and Estradiol-dependent Transcription Activation J. Biol. Chem., September 27, 1996; 271(39): 24172 - 24178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Metzger, S. Ali, J.-M. Bornert, and P. Chambon Characterization of the Amino-terminal Transcriptional Activation Function of the Human Estrogen Receptor in Animal and Yeast Cells J. Biol. Chem., April 21, 1995; 270(16): 9535 - 9542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kobayashi, T. Kitamoto, Y. Masuhiro, M. Watanabe, T. Kase, D. Metzger, J. Yanagisawa, and S. Kato p300 Mediates Functional Synergism between AF-1 and AF-2 of Estrogen Receptor alpha and beta by Interacting Directly with the N-terminal A/B Domains J. Biol. Chem., May 19, 2000; 275(21): 15645 - 15651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Routledge, R. White, M. G. Parker, and J. P. Sumpter Differential Effects of Xenoestrogens on Coactivator Recruitment by Estrogen Receptor (ER) alpha and ERbeta J. Biol. Chem., November 10, 2000; 275(46): 35986 - 35993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Heery, S. Hoare, S. Hussain, M. G. Parker, and H. Sheppard Core LXXLL Motif Sequences in CREB-binding Protein, SRC1, and RIP140 Define Affinity and Selectivity for Steroid and Retinoid Receptors J. Biol. Chem., February 23, 2001; 276(9): 6695 - 6702. [Abstract] [Full Text] [PDF]
|
|