Skip Navigation

This Article
Right arrow Print PDF (729K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Fong, S.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fong, S.-L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 11 2865-2870
© 1992

MOLECULAR BIOLOGY

Characterization of the human rod transducin alpha-subunit gene

Shao-Ling Fong

Departments of Ophthalmology, Biochemistry and Molecular Biology, Indiana University Indianapolis, IN 46202, USA

Received January 14, 1992. Revised April 20, 1992. Accepted April 20, 1992.

The human rod transducin {alpha} subunit (Tr{alpha}) gene has been cloned. A cDNA clone, HG14, contained a 1.1 kb insertion when compared with the human Tr{alpha} cDNA published by Van Dop et al. (1). Based on two overlapping clones isolated from a human genomic library, the human Tr{alpha} gene is 4.9 kb in length and consists of nine exons interrupted by eight introns. Northern blots of human retina total RNA showed that the gene is transcribed by rod photoreceptors into two species of mRNA, 1.3 kb and 2.4 kb in size. Apparently, this is the result of alternative splicing. Two putative transcription initiation sites were determined by primer extension and S1 nuclease protection assays. The putative promoter regions of the human and mouse Tr{alpha} genes have an identity of 78.1%. As found in the mouse gene (2), no TATA consensus sequence is present in the human gene.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Br. J. Ophthalmol.Home page
M Michaelides, I A Aligianis, G E Holder, M Simunovic, J D Mollon, E R Maher, D M Hunt, and A T Moore
Cone dystrophy phenotype associated with a frameshift mutation (M280fsX291) in the {alpha}-subunit of cone specific transducin (GNAT2)
Br. J. Ophthalmol., November 1, 2003; 87(11): 1317 - 1320.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
I A Aligianis, T Forshew, S Johnson, M Michaelides, C A Johnson, R C Trembath, D M Hunt, A T Moore, and E R Maher
Mapping of a novel locus for achromatopsia (ACHM4) to 1p and identification of a germline mutation in the {alpha} subunit of cone transducin (GNAT2)
J. Med. Genet., September 1, 2002; 39(9): 656 - 660.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.