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Nucleic Acids Research, 1992, Vol. 20, No. 12 2941-2945
© 1992

MOLECULAR BIOLOGY

Differential binding of human nuclear proteins to Alu subfamilies

N.V. Tomilin, V.M. Bozhkov, E.M. Bradbury1 and C.W. Schmid2,*

Institute of Cytology, The Academy of Sciences of the USSR Leningrad 194064, Russia 1Department of Biological Chemistry, University of California Davis, CA 95616, USA 2Departments of Genetics and Chemistry, University of California Davis, CA 95616, USA

* To whom correspondence should be addressed

Received April 22, 1992. Accepted May 12, 1992.

Several diagnostic differences that distinguish human Alu subfamilies are clustered just downstream from the B box of the RNA polymerase III promoter; we tentatively refer to this diagnostic region as the DB box. Assuming that this region might determine the relative transcriptional activity of Alu subfamilies, we examined the interaction of nuclear proteins with DB box sequences representing different Alu subfamilies. Gel mobility shift assays suggest the existence of two factors which discriminate among the DB boxes of different Alu subfamilies: 1) An abundant, ca. 50 kd, protein binds more stably to a young ‘PV’ Alu subfamily (PVS) than to the older major subfamily (MS). 2) Methylation of CpG dinucleotides stimulates the binding of a less abundant, ca. 70 kd, protein to the DB boxes of younger Alu subfamilies.


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