Skip Navigation

This Article
Right arrow Print PDF (5165K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Luebke, K. J.
Right arrow Articles by Dervan, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luebke, K. J.
Right arrow Articles by Dervan, P. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 12 3005-3009
© 1992

MOLECULAR BIOLOGY

Nonenzymatic ligation of double-helical DNA by alternate-strand triple helix formation

Kevin J. Luebke and Peter B. Dervan*

Arnold and Mabel Beckman Laboratory of Chemical Synthesis, California Institute of Technology Pasadena, CA 91125, USA

* To whom correspondence should be addressed

Received April 1, 1992. Revised May 15, 1992. Accepted May 15, 1992.

Nonenzymatic ligation of double-stranded DNA has been performed using an alternate-strand binding oligodeoxyribonucleotide template to juxtapose the duplex termini in a triple helical complex. The template associates with the duplex termini by Hoogsteen hydrogen bonding to alternate strands on opposite sides of the ligation site. Intermolecular and intramolecular ligation of linearized plasmid DNA are observed in the reaction, which depends on the template oligodeoxyribonucleotide and a condensing agent, N-cyanoimidazole. Intramolecular ligation products include those in which both strands are covalently closed in a circle. Ligation of the two strands is sequential and occurs at comparable rates for the first and second strands ligating. The covalent linkages formed in the reaction can be cleaved by the restriction endonuclease Stu I, supporting their identification as phosphodiesters.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Qi and R. H. Shafer
Covalent ligation studies on the human telomere quadruplex
Nucleic Acids Res., June 2, 2005; 33(10): 3185 - 3192.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Zhou, G. Chen, Y. Wang, Q. Zhang, M. Yang, and T. Li
Synthesis of unimolecularly circular G-quadruplexes as prospective molecular probes
Nucleic Acids Res., December 8, 2004; 32(21): e173 - e173.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.