Skip Navigation

This Article
Right arrow Print PDF (871K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tanaka, S.
Right arrow Articles by Isono, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanaka, S.
Right arrow Articles by Isono, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 12 3011-3020
© 1992

MOLECULAR BIOLOGY

Physical dissection and characterization of chromosomes V and VIII of Saccharomyces cerevisiae

Seiji Tanaka1 and Katsumi Isono1,2,*

1Division of Science of Intelligence, Postgraduate School of Science and Technology Rokkodai, Kobe 657, Japan 2Department of Biology, Faculty of Science, Kobe University Rokkodai, Kobe 657, Japan

* To whom correspondence should be addressed

Received April 1, 1992. Revised May 7, 1992. Accepted May 7, 1992.

Chromosomes V and VIII of S.cerevisiae were dissected and ordered clone banks were constructed and characterized. Each bank contains almost the entire chromosome from the left to the right telomere except for a small gap in each case. The size of the banks constructed is in good agreement with the physical length of these chromosomes, 580 kb, estimated by pulsed-field gel electrophoresis. The remaining gap in the ordered clone bank of chromosome V was found to be only 1.6 kb in length and to contain a 1.5 kb-long portion of one of the two Ty elements located in tandem. The gap in the bank of chromosome VIII was 6.4 kb in length and contained four copies of the CUP1 gene. A genomic restriction map analysis of the corresponding region of chromosome VIII revealed that a unit of about 2 kb in length harbouring the CUP1 gene was repeated ten times in strain DC5{varrho}° which was used for the bank construction. A 588.5 kb-long high resolution physical map for chromosome V and a 585.6 kb-long one for chromosome VIII have thus been established.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J.-M. Camadro and P. Labbe
Cloning and Characterization of the Yeast HEM14 Gene Coding for Protoporphyrinogen Oxidase, the Molecular Target of Diphenyl Ether-type Herbicides
J. Biol. Chem., April 12, 1996; 271(15): 9120 - 9128.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.