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Nucleic Acids Research, 1992, Vol. 20, No. 12 3043-3049
© 1992

MOLECULAR BIOLOGY

Extension of the DNA binding consensus of the chicken c-Myb and v-Myb proteins

Kathleen Weston

Institute of Cancer Research, Chester Beatty Laboratories 237 Fulham Road, London SW3 6JB, UK

Received March 17, 1992. Revised May 26, 1992. Accepted May 26, 1992.

The chicken c-myb gene and the v-myb oncogene transduced by avian myeloblastosis virus (AMV) encode DNA binding transcription activators. The DNA binding domain of AMV v-Myb displays a number of amino acid changes relative to c-Myb; v-Myb proteins in which one or more of three crucial residues in the DNA binding domain are mutated to resemble the c-Myb sequence display altered transformation phenotypes. In order to establish whether the spectrum of DNA binding sites which AMV v-Myb can recognise is different from that seen by chicken c-Myb, a site selection protocol was used to derive consensus binding sequences for three variant Myb proteins made in vitro, and also using nuclear extract from the v-myb transformed cell line BM2. The results show that the original consensus binding site defined for v-Myb, YAA-CKG, can be extended to YAACKGHH, and that this new consensus holds for both v-Myb and chicken c-Myb.


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