Nucleic Acids Research, 1992, Vol. 20, No. 12 3091-3098
© 1992
MOLECULAR BIOLOGY |
Molecular cloning of two C/EBP-related proteins that bind to the promoter and the enhancer of the 
1-fetoprotein gene. Further analysis of C/EBPß and C/EBP
Le Centre de Recherche en Cancérologie de I'Université Laval, L'Hôtel-Dieu de Québec Québec G1R 2J6, Canada
* To whom correspondence should be addressed
Received March 6, 1992. Revised May 7, 1992. Accepted May 7, 1992.
In an attempt to identify proteins that may regulate 
1-fetoprotein (AFP) gene expression, we screened a cDNA expression library from neonatal rat liver with two essential cis-elements of the AFP promoter and enhancer. We isolated two cDNAs which were found to correspond to leucine zipper proteins of the CC-AAT/enhancer binding protein (C/EBP) family: C/EBPß and C/EBP
. The three related proteins C/EBP , ß and bind with indistinguishable specificity to multiple DNA sites in the promoter and the enhancer of the AFP gene. In addition, C/EBPß and C/EBP readily heterodimerize with each other as well as with C/EBP. The mRNAs coding for C/EBPß and C/EBP are expressed in a wider variety of rat tissues than C/EBP mRNA, including yolk sac and fetal liver. The steady-state levels of C/EBP , ß and mRNAs increase during liver development, in parallel with their respective gene transcriptional rates. The high levels of C/EBP ß and mRNAs in rat yolk sac and fetal liver, where C/EBP is poorly expressed, suggest that C/EBP ß and/or could be preponderant or early regulators of the AFP gene in these tissues.
+ Present address: Centre de Recherche sur I'Endocrinologie Moléculaire et le Développement, CNRS, UPR 1511, 9 rue Jules Hetzel, 92190 Meudon, France
* Present address: Département de Biochimie, Faculté des Sciences et Génie, Université Laval, Québec, Canada
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