Nucleic Acids Research, 1992, Vol. 20, No. 13 3367-3373
© 1992
MOLECULAR BIOLOGY |
Functional elements of the ribosomal protein L7a (rpL7a) gene promoter region and their conservation between mammals and birds
Eukaryotic Gene Organization and Expression Laboratory, Imperial Cancer Research Fund PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK
* To whom correspondence should be addressed
Received April 3, 1992. Revised June 2, 1992. Accepted June 2, 1992.
The transcriptional initiation sites of the chicken ribosomal protein L7a (rpL7a) gene have been determined and found to occur at three consecutive cytidine residues at the start of a polypyrimidine tract of 8 base pairs (bp). A comparative analysis of the 5' upstream regions of the mouse, human and chicken rpL7a genes identified two sequence elements (Box A and Box B) conserved over the 600 million years of divergent evolution that separate mammals and birds. Only Box A (nts –56 to –39) and Box B (nts –25 to –4) sequences were detected to bind nuclear factors from mouse nuclear extracts in an analysis of the mouse rpL7a 5' upstream sequence. Box A and Box B bind different nuclear factors and the factor binding to mouse Box A and mouse Box B sequences could be effectively competed by corresponding homologous sequences from the human and chicken rpL7a promoters. These results indicate that elements of the rpL7a promoter region are conserved between mammals and birds. An in vivo analysis of the mouse rpL7a 5' upstream sequence required for efficient transcription identified the 5' border of the minimal promoter region as lying between nts –50 and –56. Constructs containing 56 bp of 5' upstream DNA and the first 25 bp rpL7a exon were very efficiently transcribed indicating that sequences within the first intron are not required for gene expression. No sequence similarity was detected between the rpL7a promoter elements and described promoter elements of other eukaryotic ribosomal protein genes.
+ Present address: Instituto di Biologia dello Sviluppo del Coasiglio Nazionale delle Ricerche, Via Archirafi 20, 90123 Palermo, Italy
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