Skip Navigation

This Article
Right arrow Print PDF (5841K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (28)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tarnura, K.
Right arrow Articles by Fukamizu, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tarnura, K.
Right arrow Articles by Fukamizu, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 14 3617-3623
© 1992

MOLECULAR BIOLOGY

A combination of upstream and proximal elements is required for effecient expression of the mouse renin promoter in cultured cells

Kouichi Tarnura, Keiji Tanimoto, Kazuo Murakami and Akiyoshi Fukamizu*

Institute of Applied Biochemistry, University of Tsukuba Tsukuba, Ibaraki 305, Japan

*To whom correspondence should be addressed

Received April 15, 1992. Accepted June 22, 1992.

Renin, a key enzyme controlling blood pressure, is produced mainly in the kidney. To identify the transcriptional regulatory elements of the mouse Ren-1c gene, the promoter regions were fused to the CAT reporter gene and transfected into embryonic kidney-derived 293 cells and four extrarenal cell lines, HeLa, HepG2, HT1080 and NIH3T3 cells. Transient transfection assay showed that sequences from – 365 to +16 of the renin gene could direct transcription of the CAT hybrid gene only in 293 cells. Deletion analysis identified two transcriptlonally active regions; the renin upstream–promoter element (RU–1 element; position –224 to –138) and the renin proximal–promoter element (RP–2 element; position –75 to –47). Although the RU–1 element functioned as an activator, depending on its orientation, it failed to trans-activate the renin promoter when the RP–2 element was deleted. By contrast, the proximal element alone exhibited a weak trans-activator property. Gel shift assay identified RU–1 element-binding factors in both 293 and HeLa cells, whereas 293 cell-dominant factors were shown to bind only to RP–2 element. Therefore, both RU–1 and RP–2 elements were found to be necessary for efficient CAT expression from the renin promoter in 293 cells, suggesting that activation of the Ren–1c promoter requires combined action between cell type–dominant and ubiquitous nuclear factors.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Choudhary, A. Palm-Leis, R. C. Scott III, R. S. Guleria, E. Rachut, K. M. Baker, and J. Pan
All-trans retinoic acid prevents development of cardiac remodeling in aortic banded rats by inhibiting the renin-angiotensin system
Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H633 - H644.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Pan, C. A. Jones, S. T. Glenn, and K. W. Gross
Identification of a novel region in the proximal promoter of the mouse renin gene critical for expression
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1107 - F1115.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
T. Voigtlander, D. Ganten, and M. Bader
Transcriptional Regulation of the Rat Renin Gene by Regulatory Elements in Intron I
Hypertension, January 1, 1999; 33(1): 303 - 311.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Tamura, S. Umemura, N. Nyui, S. Yamaguchi, T. Ishigami, K. Hibi, M. Yabana, M. Kihara, A. Fukamizu, K. Murakami, et al.
A Novel Proximal Element Mediates the Regulation of Mouse Ren-1C Promoter by Retinoblastoma Protein in Cultured Cells
J. Biol. Chem., July 4, 1997; 272(27): 16845 - 16851.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Petrovic, T. A. Black, J. R. Fabian, C. Kane, C. A. Jones, J. A. Loudon, J. P. Abonia, C. D. Sigmund, and K. W. Gross
Role of Proximal Promoter Elements in Regulation of Renin Gene Transcription
J. Biol. Chem., September 13, 1996; 271(37): 22499 - 22505.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.