Skip Navigation

This Article
Right arrow Print PDF (6243K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Daugeron, M.-C.
Right arrow Articles by Cathola, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Daugeron, M.-C.
Right arrow Articles by Cathola, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 14 3625-3630
© 1992

MOLECULAR BIOLOGY

U1—U2 snRNPs interaction induced by an RNA complementary to the 5' end sequence of U1 snRNA

Marie-Claire Daugeron, Jamal Tazi, Philippe Jeanteur, Claude Brunel* and Guy Cathola

UA CNRS 1191, Génétique Moléculaire, Laboratoire de Biochimie, CRLC Val d'Aurelle-Paul Lamarque, Parc Euromédecine, 34094 Montpellier Cedex 2 and Laboratoire de Biologie Moléculaire, Université Montpellier II, Sciences et Techniques du Languedoc Place E. Bataillon, CP 012, 34095 Montpellier Cedex 05, France

*To whom correspondence should be addressed at: UA CNRS 1191, Géneuque Moléculaire, Université Montpellier II, Sciences et Techniques du Languedoc, Place E. Bataillon, CP 012, 34095 Montpellier Cedex 05, France

Received April 8, 1992. Accepted June 25, 1992.

Several lines of evidences indicate that U1 and U2 snRNPs become interacting during pre-mRNA splicing. Here we present data showing that an U1 – U2 snRNPs interaction can be mediated by an RNA only containing the consensus 5' splice site of all of the sequences characteristic of pre-mRNAs. Using monospecific antibodies (anti-(U1) RNP and anti-(U2) RNP), we have found that a tripartite complex comprising U1 and U2 snRNPs is immunoprecipitated in the presence of a consensus 5' splice site containing RNA, either from a crude extract or from an artificial mixture enriched in U1 and U2 snRNPs. This complex does not appear in the presence of an RNA lacking the sequence complementary to the 5' terminus of U1 snRNA. Moreover, RNAse T1 protection coupled to immunoprecipltation experiments have demonstrated that only the 5' end sequence of U1 snRNA contacts the consensus 5' splice site containing RNA, arguing that U2 snRNP binding in the tripartite complex is mediated by U1 snRNP.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
B. K. Abu Dayyeh, T. K. Quan, M. Castro, and S. W. Ruby
Probing Interactions between the U2 Small Nuclear Ribonucleoprotein and the DEAD-box Protein, Prp5
J. Biol. Chem., June 7, 2002; 277(23): 20221 - 20233.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Ast, T. Pavelitz, and A. M. Weiner
Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction
Nucleic Acids Res., April 15, 2001; 29(8): 1741 - 1749.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
J. Zhao, L. Hyman, and C. Moore
Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis
Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 405 - 445.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. Ruby
Dynamics of the U1 Small Nuclear Ribonucleoprotein during Yeast Spliceosome Assembly
J. Biol. Chem., July 11, 1997; 272(28): 17333 - 17341.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Rossi, T. Forne, E. Antoine, J. Tazi, C. Brunel, and G. Cathala
Involvement of U1 Small Nuclear Ribonucleoproteins (snRNP) in 5' Splice Site-U1 snRNP Interaction
J. Biol. Chem., September 27, 1996; 271(39): 23985 - 23991.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
K M Wassarman and J A Steitz
Association with terminal exons in pre-mRNAs: a new role for the U1 snRNP?
Genes & Dev., April 1, 1993; 7(4): 647 - 659.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.