Skip Navigation

This Article
Right arrow Print PDF (5519K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Harper, D. S.
Right arrow Articles by Keene, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harper, D. S.
Right arrow Articles by Keene, J. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 14 3645-3650
© 1992

MOLECULAR BIOLOGY

RNA binding specificity of a Drosophila snRNP protein that shares sequence homology with mammalian U1-A and U2-B’ proteins

David S. Harper, Lucille D. Fresco+ and Jack D. Keene*

Department of Microbiology and Immunology, Duke University Medical Center Durham, NC 27710 USA

*To whom correspondence should be addressed

Received April 1, 1992. Accepted June 15, 1992.

We have characterized a recomblnant Drosophila melanogaster RNA binding protein, D25, by virtue of its antlgenic relationship to mammalian U1 and U2 small nuclear ribonucleoprotein (U snRNP) proteins. Sequence analysis revealed that D25 bears strong similarity to both the human U1 snRNP-A (U1-A) and U2 snRNP-B’ (U2-B’) proteins. However, at residues known to be critical for the RNA binding specificities of U1-A and U2-B’ D25 sequence is more similar to U2-B’. Using direct RNA binding assays D25 selected U1 RNA from either HeLa or Drosophila Kc cell total RNA. Furthermore, D25 bound U1 RNA when transfected into mammalian cells. Thus, D25 appears to be a Drosophila homolog of the mammalian U1-A protein, despite its sequence similarity to U2-B’.

+Present address: Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
C. Preusser, Z. Palfi, and A. Bindereif
Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei
Eukaryot. Cell, August 1, 2009; 8(8): 1228 - 1234.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
L. O. F. Penalva and L. Sanchez
RNA Binding Protein Sex-Lethal (Sxl) and Control of Drosophila Sex Determination and Dosage Compensation
Microbiol. Mol. Biol. Rev., September 1, 2003; 67(3): 343 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. A. Nagengast and H. K. Salz
The Drosophila U2 snRNP protein U2A' has an essential function that is SNF/U2B'' independent
Nucleic Acids Res., September 15, 2001; 29(18): 3841 - 3847.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
G Deshpande, G Calhoun, and P. Schedl
The N-terminal domain of Sxl protein disrupts Sxl autoregulation in females and promotes female-specific splicing of tra in males
Development, January 7, 1999; 126(13): 2841 - 2853.
[Abstract] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. Meyer, B. Oliver, and D. Pauli
Multiple Developmental Requirements of Noisette, the Drosophila Homolog of the U2 snRNP-Associated Polypeptide SF3a60
Mol. Cell. Biol., April 1, 1998; 18(4): 1835 - 1843.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. Wang, Z. Dong, and L. R. Bell
Sex-lethal Interactions with Protein and RNA. ROLES OF GLYCINE-RICH AND RNA BINDING DOMAINS
J. Biol. Chem., August 29, 1997; 272(35): 22227 - 22235.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T W Flickinger and H K Salz
The Drosophila sex determination gene snf encodes a nuclear protein with sequence and functional similarity to the mammalian U1A snRNP protein.
Genes & Dev., April 15, 1994; 8(8): 914 - 925.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.