Nucleic Acids Research, 1992, Vol. 20, No. 14 3671-3678
© 1992
MOLECULAR BIOLOGY |
hnRNP 1, the polyprimidine tract-binding protein: distinct nuclear localization and association with hnRNAs
1Universtia di Verona, Facolta di Medicina e Chirurgia, Instito di Scienze Biologiche 37134 Verona Strade Le Grazie 2IGBE, CNR Pavia, Italy 3Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine Philadelphia, PA 19104–6148, USA
*To whom correspondence should be addressed
Received March 25, 1992. Revised May 27, 1992. Accepted May 27, 1992.
Many hnRNP proteins and snRNPs Interact with hnRNA in the nucleus of eukaryotic cells and affect the fate of hnRNA and its processing into mRNA. There are at least 20 abundant proteins in vertebrate cell hnRNP complexes and their structure and arrangement on specific hnRNAs is likely to be important for the processing of pre-mRNAs. hnRNP I, a basic protein of ca. 58,000 daltons by SDS-PAGE, is one of the abundant hnRNA-binding proteins. Monoclonal antibodies to hnRNP I were produced and full length cDNA clones for hnRNP I were isolated and sequenced. The sequence of hnRNP I (59,632 daltons and pi 9.86) demonstrates that it is identical to the previously described polypyrimidine tract-binding protein (PTB) and shows that it is highly related to hnRNP L. The sequences of these two proteins, I and L, define a new family of hnRNP proteins within the large superfamily of the RNP consensus RNA-binding proteins. Here we describe experiments which reveal new and unique properties on the association of hnRNP I/PTB with hnRNP complexes and on its cellular localization. Micrococcal nuclease digestions show that hnRNP I, along with hnRNP S and P, is released from hnRNP complexes by nuclease digestion more readily than most other hnRNP proteins. This nuclease hypersensitivity suggests that hnRNP I is bound to hnRNA regions that are particularly exposed in the complexes. Immunofiuorescence microscopy shows that hnRNP I is found In the nucleoplasm but in addition high concentrations are detected in a discrete perinucleolar structure. Thus, the PTB is one of the major proteins that bind pre-mRNAs; it is bound to nuclease-hypersensitive regions of the hnRNA-protein complexes and shows a novel pattern of nuclear localization.
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|
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|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
 S. Huang, T. J. Deerinck, M. H. Ellisman, and D. L. Spector The Perinucleolar Compartment and Transcription J. Cell Biol., October 5, 1998; 143(1): 35 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Sun, A. T. Alzhanova-Ericsson, N. Visa, Y. Aissouni, J. Zhao, and B. Daneholt The hrp23 Protein in the Balbiani Ring Pre-mRNP Particles Is Released Just before or at the Binding of the Particles to the Nuclear Pore Complex J. Cell Biol., September 7, 1998; 142(5): 1181 - 1193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Schul, R. van Driel, and L. de Jong Coiled Bodies and U2 snRNA Genes Adjacent to Coiled Bodies Are Enriched in Factors Required for snRNA Transcription Mol. Biol. Cell, May 1, 1998; 9(5): 1025 - 1036. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. I. Lamond and W. C. Earnshaw Structure and Function in the Nucleus Science, April 24, 1998; 280(5363): 547 - 553. [Abstract] [Full Text]
|
|
|
|
|
|
S. Huang, T. J. Deerinck, M. H. Ellisman, and D. L. Spector The Dynamic Organization of the Perinucleolar Compartment in the Cell Nucleus J. Cell Biol., June 2, 1997; 137(5): 965 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Grande, I van der Kraan, L de Jong, and R van Driel Nuclear distribution of transcription factors in relation to sites of transcription and RNA polymerase II J. Cell Sci., January 8, 1997; 110(15): 1781 - 1791. [Abstract] [PDF]
|
|
|
|
|
|
H. Inagaki, Y. Matsushima, K. Nakamura, M. Ohshima, T. Kadowaki, and Y. Kitagawa A Large DNA-binding Nuclear Protein with RNA Recognition Motif and Serine/Arginine-rich Domain J. Biol. Chem., May 24, 1996; 271(21): 12525 - 12531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Czyzyk-Krzeska and J. E. Beresh Characterization of the Hypoxia-inducible Protein Binding Site within the Pyrimidine-rich Tract in the 3`-Untranslated Region of the Tyrosine Hydroxylase mRNA J. Biol. Chem., February 9, 1996; 271(6): 3293 - 3299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X Liu and J E Mertz HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression. Genes & Dev., July 15, 1995; 9(14): 1766 - 1780. [Abstract] [PDF]
|
|
|
|
|
|
R Singh, J Valcarcel, and M. Green Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins Science, May 26, 1995; 268(5214): 1173 - 1176. [Abstract] [PDF]
|
|
|
|
|
|
I Solovei, H Macgregor, and E Gaginskaya Single stranded nucleic acid binding structures on chicken lampbrush chromosomes J. Cell Sci., January 4, 1995; 108(4): 1391 - 1396. [Abstract] [PDF]
|
|
|
|
|
|
P. Norton Alternative pre-mRNA splicing: factors involved in splice site selection J. Cell Sci., January 1, 1994; 107(1): 1 - 7. [PDF]
|
|
|
|
|
|
J R Wyatt, E J Sontheimer, and J A Steitz Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing. Genes & Dev., December 1, 1992; 6(12b): 2542 - 2553. [Abstract] [PDF]
|
|
|
|
|
|
S. Runge, F. C. Nielsen, J. Nielsen, J. Lykke-Andersen, U. M. Wewer, and J. Christiansen H19 RNA Binds Four Molecules of Insulin-like Growth Factor II mRNA-binding Protein J. Biol. Chem., September 15, 2000; 275(38): 29562 - 29569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Giraud, A. Greco, M. Brink, J.-J. Diaz, and P. Delafontaine Translation Initiation of the Insulin-like Growth Factor I Receptor mRNA Is Mediated by an Internal Ribosome Entry Site J. Biol. Chem., February 16, 2001; 276(8): 5668 - 5675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cote, S. Dupuis, and J. Y. Wu Polypyrimidine Track-binding Protein Binding Downstream of Caspase-2 Alternative Exon 9 Represses Its Inclusion J. Biol. Chem., March 9, 2001; 276(11): 8535 - 8543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Fabini, R. Raijmakers, S. Hayer, M. A. Fouraux, G. J. M. Pruijn, and G. Steiner The Heterogeneous Nuclear Ribonucleoproteins I and K Interact with a Subset of the Ro Ribonucleoprotein-associated Y RNAs in Vitro and in Vivo J. Biol. Chem., June 1, 2001; 276(23): 20711 - 20718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Polydorides, H. J. Okano, Y. Y. L. Yang, G. Stefani, and R. B. Darnell A brain-enriched polypyrimidine tract-binding protein antagonizes the ability of Nova to regulate neuron-specific alternative splicing PNAS, June 6, 2000; 97(12): 6350 - 6355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|