Nucleic Acids Research, 1992, Vol. 20, No. 14 3701-3712
© 1992
MOLECULAR BIOLOGY |
The cooperative interaction between two motifis of an enhancer element of the chicken 
A-crystallin gene, CE1 and CE2, confers lens-specific expression
Department of Biophysics, Faculty of Science, Kyoto University Kyoto 606–01, Japan
*To whom correspondence should be addressed
Received March 20, 1992. Revised June 17, 1992. Accepted June 17, 1992.
An 84 bp element located between nucleotides –162 and –79 of the chicken aA-crystallin gene exhibits lens-specific enhancer activity. Transient transfection experiments using 5' deletion and linker scanner mutants has indicated that the 84 bp enhancer element is composed of three motifs, 
CE1 (–162 to –134), CE3(–135 to –121)andoCE2(–119 to –99). Neither CE1 or CE3 motif alone can exhibit enhancer activity even when trimerized, whereas together they can direct some degree of lens-specific expression. CE2 alone shows low transcriptlonal activity when trimerized. A combination of CE1 with CE2 exerts full lens-specific enhancer activity comparable with that of the 84 bp enhancer element, indicating that CE1 and CE2 motifs are sufficient to confer lens-specific expression. Transcriptional activation by these two motifs from a distance required the additional presence of either or both motifs adjacent to the ß-actin basal promoter. Gel shift experiments indicated that the CE1, CE2 and CE3 motifs specifically bind nuclear proteins. CE1 binds a protein predominantly present in lens cells, whereas CE2- and CE3-binding proteins differ between lens and lung cells. Mutations within the CE1 and CE2 motifs that failed to bind nuclear factors in vitro resulted in loss of transcriptional activation, indicating that these nuclear factors play a key role in controlling lens-specific expression.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Yoshida, T. Ohkumo, S. Ishibashi, and K. Yasuda The 5'-AT-rich half-site of Maf recognition element: a functional target for bZIP transcription factor Maf Nucleic Acids Res., June 21, 2005; 33(11): 3465 - 3478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Carosa, Z. Kozmik, J. E. Rall, and J. Piatigorsky Structure and Expression of the Scallop Omega -Crystallin Gene. EVIDENCE FOR CONVERGENT EVOLUTION OF PROMOTER SEQUENCES J. Biol. Chem., January 4, 2002; 277(1): 656 - 664. [Abstract] [Full Text]
|
|
|
|
|
|
M. Sakai, M. S. Serria, H. Ikeda, K. Yoshida, J. Imaki, and S. Nishi Regulation of c-maf gene expression by Pax6 in cultured cells Nucleic Acids Res., March 1, 2001; 29(5): 1228 - 1237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ring, S. Cordes, P. Overbeek, and G. Barsh Regulation of mouse lens fiber cell development and differentiation by the Maf gene Development, January 1, 2000; 127(2): 307 - 317. [Abstract] [PDF]
|
|
|
|
|
|
S. Kawauchi, S. Takahashi, O. Nakajima, H. Ogino, M. Morita, M. Nishizawa, K. Yasuda, and M. Yamamoto Regulation of Lens Fiber Cell Differentiation by Transcription Factor c-Maf J. Biol. Chem., July 2, 1999; 274(27): 19254 - 19260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Kim, T. Li, I.-C. Ho, M. J. Grusby, and L. H. Glimcher Requirement for the c-Maf transcription factor in crystallin gene regulation and lens development PNAS, March 30, 1999; 96(7): 3781 - 3785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ogino and K. Yasuda Induction of Lens Differentiation by Activation of a bZIP Transcription Factor, L-Maf Science, April 3, 1998; 280(5360): 115 - 118. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Sharon-Friling, J. Richardson, S. Sperbeck, D. Lee, M. Rauchman, R. Maas, A. Swaroop, and G. Wistow Lens-Specific Gene Recruitment of zeta -Crystallin through Pax6, Nrl-Maf, and Brain Suppressor Sites Mol. Cell. Biol., April 1, 1998; 18(4): 2067 - 2076. [Abstract] [Full Text]
|
|
|
|
|
|
K. Kataoka, S. Shioda, K. Yoshitomo-Nakagawa, H. Handa, and M. Nishizawa Maf and Jun Nuclear Oncoproteins Share Downstream Target Genes for Inducing Cell Transformation J. Biol. Chem., September 21, 2001; 276(39): 36849 - 36856. [Abstract] [Full Text] [PDF]
|
|