Skip Navigation

This Article
Right arrow Print PDF (3730K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Willis, I.
Right arrow Articles by López-De-León, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Willis, I.
Right arrow Articles by López-De-León, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 14 3725-3730
© 1992

MOLECULAR BIOLOGY

The PCF1–1 mutation increases the activity of the transcription factor (TF) IIIB fraction from Saccharomyces cerevisae

Ian Willis*, Alexander Oksman+ and Alfredo López-De-León

Department of Biochemistry, Albert Einstein College of Medicine, Jack and Pearl Resnick Campus 1300 Morris Park Avenue, Bronx, NY 10461, USA

*To whom correspondence should be addressed

Received March 13, 1992. Revised June 8, 1992. Accepted June 8, 1992.

PCF1–1 is a dominant suppressor of a tRNA gene A block promoter mutation (A19) In Saccharomyces cerevisiae. Transcriptlonal activation by PCF1–1 was examined in vitro using whole-cell extracts and purified factors derived from mutant and wild-type strains. These experiments show that PCF1 Is a general activator of RNA polymerase III (pol III) gene transcription. The transcription of all pol III genes analyzed to date, including type I and numerous type II genes, is increased 3 – 7 fold In mutant cell extracts. Single round transcription assays indicate that the PCF1–1 mutation increases the number of functional preinltiation complexes and suggest that this is achieved by increasing the intrinsic activity of the encoded product rather than its amount. Point mutations throughout the A block of the sup3-e gene and numerous B block mutations fall to abolish transcrlptional activation suggesting that interactions between TFIIIC and the internal promoter are unaffected by PCF1–1. Moreover, TFIIIC purified from the mutant strain Is incapable of conferring PCF1–1 transcrlptional activity to a reaction in which the remaining components are wild-type. In contrast, the activity of the TFIIIB fraction is increased in PCF1–1 extracts and can reconstitute mutant levels of transcription when added to wild-type TFIIIC and polymerase. We conclude that PCF1 is a component or regulator of TFIIIB.

+Present address: Waisman Center, University of Wisconsin-Madison, 1500 Highland Averue, Madison, WI 53705, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
R. D. Moir, K. V. Puglia, and I. M. Willis
A Gain-of-Function Mutation in the Second Tetratricopeptide Repeat of TFIIIC131 Relieves Autoinhibition of Brf1 Binding
Mol. Cell. Biol., September 1, 2002; 22(17): 6131 - 6141.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
R. Arrebola, N. Manaud, S. Rozenfeld, M.-C. Marsolier, O. Lefebvre, C. Carles, P. Thuriaux, C. Conesa, and A. Sentenac
tau 91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with tau 138 in DNA Binding
Mol. Cell. Biol., January 1, 1998; 18(1): 1 - 9.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. D. Librizzi, R. D. Moir, M. Brenowitz, and I. M. Willis
Expression and Purification of the RNA Polymerase III Transcription Specificity Factor IIIB70 from Saccharomyces cerevisiae and Its Cooperative Binding with TATA-binding Protein
J. Biol. Chem., December 20, 1996; 271(51): 32695 - 32701.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Sethy, R. D. Moir, M. Librizzi, and I. M. Willis
In Vitro Evidence for Growth Regulation of tRNA Gene Transcription in Yeast
J. Biol. Chem., November 24, 1995; 270(47): 28463 - 28470.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.