Skip Navigation

This Article
Right arrow Print PDF (2472K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (67)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Deursen, J. V.
Right arrow Articles by Wieringa, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deursen, J. V.
Right arrow Articles by Wieringa, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 15 3815-3820
© 1992

MOLECULAR BIOLOGY

Targeting of the creating kinase M gene in embryonic stem cells using isogenic and nonisogenic vectors

Jan Van Deursen and Beè Wieringa*

Department of Cell biology and histology, Faculty of Medicine Sciences, University of Nijmegen PO Box 9101, 6500 HB Nijmgen, The Netherlands

*To whom correspondence should be addressed

Received June 15, 1992. Accepted July 9, 1992.

Replacement vectors with genomlc DNA originating from different mouse strains were used to introduce site-specific mutations into the creatine kinase M (CKM) gene of mouse embryonic stem (ES) cells. Here we demonstrate that in mouse strain 129-derived ES cells, the gene is at least 25-fold more efficiently targeted with an Isogenic, 129-derived vector (129-pRV8.3) than with a nonisogenic, BALB/c-specific vector (BALB/c–pRV8.3). The two targeting constructs were identical except for allellc differences which were typed by partial sequencing. These included base pair mismatches (2%) and a polymorphic [GTC]-repeat length variation. Both in separate transfectlons as well as in cotransfections with mixed vectors, homologous disruption of the CKM gene resulted uniquely from the 129-isogenic DNA. Our data confirm earlier observations on requirements for homologous recombination in pro- and eukaryotic systems and indicate that targeting of the CKM locus is highly sensitive to small sequence differences between cognate segments in the endogenous and incoming DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
D. G. Wansink, W. Peters, I. Schaafsma, R. P. M. Sutmuller, F. Oerlemans, G. J. Adema, B. Wieringa, C. E. E. M. van der Zee, and W. Hendriks
Mild impairment of motor nerve repair in mice lacking PTP-BL tyrosine phosphatase activity
Physiol Genomics, September 16, 2004; 19(1): 50 - 60.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
A. Urbach, M. Schuldiner, and N. Benvenisty
Modeling for Lesch-Nyhan Disease by Gene Targeting in Human Embryonic Stem Cells
Stem Cells, July 1, 2004; 22(4): 635 - 641.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. J. Yanez and A. C. G. Porter
A chromosomal position effect on gene targeting in human cells
Nucleic Acids Res., November 15, 2002; 30(22): 4892 - 4901.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
W. J. A. A. van den Broek, M. R. Nelen, D. G. Wansink, M. M. Coerwinkel, H. te Riele, P. J. T. A. Groenen, and B. Wieringa
Somatic expansion behaviour of the (CTG)n repeat in myotonic dystrophy knock-in mice is differentially affected by Msh3 and Msh6 mismatch-repair proteins
Hum. Mol. Genet., January 1, 2002; 11(2): 191 - 198.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. M. Vasquez, K. Marburger, Z. Intody, and J. H. Wilson
Manipulating the mammalian genome by homologous recombination
PNAS, July 17, 2001; 98(15): 8403 - 8410.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Yamashita, R. Wada, T. Sasaki, C. Deng, U. Bierfreund, K. Sandhoff, and R. L. Proia
A vital role for glycosphingolipid synthesis during development and differentiation
PNAS, August 3, 1999; 96(16): 9142 - 9147.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. R. PICCIOTTO and K. WICKMAN
Using Knockout and Transgenic Mice to Study Neurophysiology and Behavior
Physiol Rev, October 1, 1998; 78(4): 1131 - 1163.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Kawai, K. Sango, K. A. Mullin, and R. L. Proia
Embryonic Stem Cells with a Disrupted GD3 Synthase Gene Undergo Neuronal Differentiation in the Absence of b-Series Gangliosides
J. Biol. Chem., July 31, 1998; 273(31): 19634 - 19638.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D ten Berge, A Brouwer, J Korving, J. Martin, and F Meijlink
Prx1 and Prx2 in skeletogenesis: roles in the craniofacial region, inner ear and limbs
Development, January 10, 1998; 125(19): 3831 - 3842.
[Abstract] [PDF]

Home page
 J. Appl. Physiol.Home page
J. F. Watchko, M. J. Daood, G. C. Sieck, J. J. Labella, B. T. Ameredes, A. P. Koretsky, and B. Wieringa
Combined myofibrillar and mitochondrial creatine kinase deficiency impairs mouse diaphragm isotonic function
J Appl Physiol, May 1, 1997; 82(5): 1416 - 1423.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. I. Veksler, A. V. Kuznetsov, K. Anflous, P. Mateo, J. van Deursen, Bé Wieringa, and R.ée Ventura-Clapier
Muscle Creatine Kinase-deficient Mice
J. Biol. Chem., August 25, 1995; 270(34): 19921 - 19929.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.