Skip Navigation

This Article
Right arrow Print PDF (715K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (37)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kumar, P.K.R.
Right arrow Articles by Nishikawa, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kumar, P.K.R.
Right arrow Articles by Nishikawa, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 15 3919-3924
© 1992

MOLECULAR BIOLOGY

Random mutations to evaluate the role of bases at two important single-stranded regions of genomic HDV ribozyme

P.K.R. Kumar1, Young-Ah Suh1, Hirotsugu Miyashiro2, Fumiko Nishikawa1, Junji Kawakami1,3, Kazunari Taira1 and Satoshi Nishikawa1,*

1Department of Cellular and Molecular Biology, Fermentation Research Institute, Agency of Industrial Science {wedge} Technology MITI, 1-1-3 Higashi, Tsukuba Science City, Ibaraki 305 2Research Institute for WAKAN-YAKU, Toyama Medical and Pharmaceutical University Toyama 930-01 3Faculty of Pharmaceutical Sciences, Hokkaido Universtiy Sapporo 060, Japan

*To whom correpondence should be addressed

Received July 7, 1992. Revised July 7, 1992. In elucidating function of two Important single-stranded regions [SSrA (726–731 nt) and SSrB (762–766 nt)] derived mainly from three secondary structure models in genomic hepatitis delta virus (HDV) ribozyme possessing self-cleavage activity, we have constructed several random mutants at those two regions on the HDV88 molecule (683–770 nt) by oligonucleotidedirected mutagenesis. When self-cleavage activities were compared among mutants, at the region SSrA, G726 was found to play an important role during cleavage reaction since substitutions of the base to A (mutant A20) or C (mutant A16) or U (mutant A23), reduced the ribozyme activity to very low levels suggesting the importance of G726 position. C763 at SSrB region was found to play a more significant role during catalysis than G726 (at region SSrA) since any substitutions at C763 completely inactivated the ribozyme. Other bases located In these two regions could be substituted to other bases at the expense of some self-cleavage activity. The results presented here together with our previous deletion analysis indicate that these two regions may play an important role during cleavage process.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Sefcikova, M. V. Krasovska, J. Sponer, and N. G. Walter
The genomic HDV ribozyme utilizes a previously unnoticed U-turn motif to accomplish fast site-specific catalysis
Nucleic Acids Res., March 19, 2007; 35(6): 1933 - 1946.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Fiola and J.-P. Perreault
Kinetic and Binding Analysis of the Catalytic Involvement of Ribose Moieties of a trans-Acting delta Ribozyme
J. Biol. Chem., July 12, 2002; 277(29): 26508 - 26516.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Nishikawa and S. Nishikawa
Requirement for canonical base pairing in the short pseudoknot structure of genomic hepatitis delta virus ribozyme
Nucleic Acids Res., February 15, 2000; 28(4): 925 - 931.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.