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Nucleic Acids Research, 1992, Vol. 20, No. 15 4015-4020
© 1992

CHEMISTRY

Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAGTAAGCC]-Glu-OH from the nucleoprotein of Bacillus Subtilis phage ø29

C.M. Dreef-Tromp, J.C.M. van der Maarel, H. van den Elst, G.A. van der Marel and J.H. van Boom*

Gorlaeus Laboratories PO Box 9502, 2300 RA Leiden, The Netherlands

*To whom Correspondence should be addressed

Received July 6, 1992. The naturally occurring DNA-nucleopeptide H-Asp-Ser[5'-pAAAGTAAGCC-3']-Glu-OH was prepared via a solid-phase phosphite triester approach using A/-2-(fert-butyldiphenylsilyloxymetriy1)benzoy1 protected nucleosides. The oligonucleotide was linked via the extremely base-labile oxalyl ester anchor to the solid support.


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