Nucleic Acids Research, 1992, Vol. 20, No. 15 4069-4076
© 1992
MOLECULAR BIOLOGY |
Intron 5
of the COXI gene of yeast mitochondrial DNA is a mobile group I intron
Department of Biochemistry, University of Texas Southwestern Medical Center 5323 Harry Hines Blvd, Dallas, TX 75235-9038 1Department of Molecular Genetics, The Ohio State University Columbus, OH 43210, USA
*To whom Correspondence should be addressed
Received March 13, 1992. Accepted June 16, 1992.
We have found that intron 5a of the COXI gene (al5
) of yeast mtDNA is a mobile group I intron in crosses between strains having or lacking the Intron. We have demonstrated the following hallmarks of that process: 1) co-conversion of flanking optional intron markers; 2) mutations that truncate the intron open reading frame block intron mobility; and 3) the intron open reading frame encodes an endonuclease activity that is required for intron movement. The endonuclease activity, termed I-Sce IV, cleaves the COXI allele lacking al5a near the site of intron insertion, making a four-base staggered cut with 3' OH overhangs. Three cloned DNAs derived from different forms of the COXI gene, which differ In primary sequence at up to seven nucleotides around the cleavage site, are all good substrates for In vitro I-Sce IV cleavage activity. Two of the strains from which these substrates were derived were tested in crosses and are comparably efficient as al5a recipients. When compared with w mobility occurring simultaneously in one cross, al5a is less efficient as a mobile element.
+Present address: Department of biological Sciences, Indiana University, South Bend, IN 46634, USA
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