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Nucleic Acids Research, 1992, Vol. 20, No. 15 4077-4081
© 1992

MOLECULAR BIOLOGY

The Mechanism of trans-activation of the Escherichia coli operon thru(tufB) by the protein FIS. A Model

Hans Verbeek, Lars Nilsson1 and Leendert Bosch*

Department of Biochemistry, Leiden University, Gorlaeus Laboratories PO Box 9502, 2300 RA Leiden, The Netherlands 1Department of Cell Biology, University of Stockholm S-106 91 Stockholm, Sweden

*To whom Correspondence should be addressed

Received March 13, 1992. Accepted June 16, 1992.

Transcription of the thrU(tufB) operon is trans-activated by the protein FIS which binds to the promoter upstream activator sequence (UAS). Deletions of parts of the UAS and insertions show that optimal trans-activation requires occupation by FIS of the two FIS-binding regions on the UAS and specific helical positioning of these regions. On the basis of these and other data, a model for the mechanism of thrtl(tufB) frans-activation by FIS Is proposed. This model implies that the mechanisms underlying stimulation by FIS of two totally different processes: inversion of viral DNA segments and transcription of stable RNA operons, are essentially the same.


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